Background: Shoulder joint is a complex joint producing wide range of motion. Joint receptors present in the capsule, ligaments transmit information about status of joint to the Central nervous system. Integrity of mechanoreceptors and CNS pathways play an important role to meet mobility and stability requirements. Receptors function to protect the joint from damage incurred by going in pathologic range of motion. Application of cryotherapy is common after acute shoulder injury as a part of rehabilitation. It is essential to study the effect of cryotherapy on functional performance of the shoulder joint and also to understand the safe recovery time post cryotherapy so as to return the subject back to activity. Materials and Methods: 50 healthy subjects (n=25 males n=25 females) were assessed using Functional Throwing Performance Index (FTPI) where the subject was told to throw the ball in a target at a measured distance using their dominant side. Then subject was given cryotherapy for 20 minutes followed by which the subject was reassessed immediately post cryotherapy, after 3min, 5min, 10min and 15min. Result: Data analysis showed a significant decrease (p<0.0001) in mean FTPI from pre-cryotherapy FTPI (0.5402 +0.1741) to post-cryotherapy FTPI (0.3787 + 0.1976). Also 3min post-cryotherapy FTPI (0.3958 + 0.1671), 5min post-cryotherapy FTPI (0.4706 + 0.1721) showed significant difference with pre-cryotherapy FTPI. Whereas, 10min post-cryotherapy FTPI (0.6063 + 0.1525) and 15min post-cryotherapy FTPI (0.6178 + 0.1466) showed no significant difference than pre-cryotherapy FTPI. Conclusion: The study suggested that functional performance of shoulder, is affected post cryotherapy till 5min, and recovers to pre values by 10min.